Knott Test:
Knott, J., 1935. The periodicity of the mf of Wuchereia bancrofti, preliminary report of some injection experiments. Trans. Roy. Soc. Trop. Med. Hyg. 29, 59–64.
In this paper Dr. James Knott described his technique for removing red blood cells and concentrating microfilariae for counting. The technique became the preferred method of testing dogs for heartworm (Dirofilaria immitis) infections before the antigen test was developed. It is still widely used in veterinary practice and is taught to every veterinary student.
https://www.sciencedirect.com/science/article/pii/S0035920335900189[Cited 36 times since 1945, while the “Knott Test” has been cited over 6000 times.]
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McMaster Counting Method:
Gordon, H. McL.; Whitlock, H.V. A new technique for counting nematode eggs in sheep faeces. Journal of the Council for Scientific and Industrial Research. 1939; 12(1):50 – 52.
In this paper Drs. Gordon and Whitlock, working at the McMaster Animal Health Laboratory in Sydney Australia, describe the counting chamber (McMaster slide) and the technique they developed for counting the number of strongyle-type eggs in a fecal sample. Used to make decisions on which animals need anthelmintics and the effectiveness of these drugs, the McMaster slide is a common piece of equipment used by equine veterinarians and horse owners.
https://publications.csiro.au/rpr/download?pid=procite:21259a33-8a8e-4add-9315-f8338091a3e6&dsid=DS1[Cited once since 1945, the technique has been referenced ~46,000 times on Google Scholar.]
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Wisconsin Counting Method:
Cox DD, Todd AC. Survey of gastrointestinal parasitism in Wisconsin dairy cattle. J Am Vet Med Assoc. 1962 Sep 15;141:706-9.
In this paper Drs. Cox and Todd, working at the University of Wisconsin, Madison, describe an egg counting technique which uses a sugar flotation to separate eggs from debris for counting. Because of the simplicity of the method, which needs only a centrifuge, their technique is still widely used where the number of eggs in a fecal sample must be determined.
https://www.cabdirect.org/cabdirect/abstract/19640800004[Cited 40 times since 1976 Wisconsin technique cited ~100 times]
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Baermann Technique:
Original Citation:
Baermann G. Eine einfache Methode zur Auffinding von Ankylostomum (Nematoden) Larven in Erdproben. Geneeskundig tijdschrift voor Nederlandsch-Indië. 1917. 57:131-137
English Translation:
Baermann G. A Simple Method for Detecting Ankylostomum (Nematodes) Larvae in Soil Samples. Medical journal for the Dutch East Indies. 1917. 57:131-137
In this paper Dr. G. Baermann describes his method of isolating hookworm larvae from soil using an apparatus consisting of a funnel and a sieve. The Baermann apparatus (Baermann technique) has become a staple of the veterinary diagnostic laboratory and is taught to every veterinary student. Used to recover live nematode larvae from feces (usually lungworms or Strongyloides stercoralis) or soil (especially by plant parasitologists) this technique has been modified in numerous ways and is so well known that when mentioned in publications a citation is only rarely given.
Paper in the Public Domain.[Cited once since 1945. Baermann technique cited 14,000 times.]
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